![]() The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. The work is made available under the Creative Commons CC0 public domain dedication.ĭata Availability: All relevant data are in the paper and its Supporting Information files.įunding: This research was supported in part by an appointment to the Visiting Scientist Program at the FBI Laboratory Division, administered by the Oak Ridge Institute of Science and Education, through an interagency agreement between the US Department of Energy and the FBI. This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. Received: JanuAccepted: Published: June 19, 2019 ![]() This study outlines the utility of the BLAST search tool in GenBank and the BOLD identification engine for taxonomic identifications and identifies some precautions needed when using public sequence repositories in applied scientific disciplines.Ĭitation: Meiklejohn KA, Damaso N, Robertson JM (2019) Assessment of BOLD and GenBank – Their accuracy and reliability for the identification of biological materials. Results illustrated that using a multi-locus barcode approach increased identification success. While GenBank outperformed BOLD for species-level identification of insect taxa (53% and 35%, respectively), both databases performed comparably for plants and macro-fungi (~81% and ~57%, respectively). To achieve this, 1) curated reference materials for plants, macro-fungi and insects were obtained from national collections, 2) relevant barcode sequences ( rbcL, matK, trnH-psbA, ITS and COI) from these reference samples were generated and used for searching against both databases, and 3) optimal search parameters were determined that ensure the best match to the known species in either database. In this study, an assessment of the accuracy and reliability of sequences in these databases was performed. The Barcode of Life DataSystems (BOLD) and GenBank are the main public repositories of DNA barcode sequences. ![]() ![]() g., evolutionary biology, food regulatory compliance, forensics). In many disciplines, obtaining accurate taxonomic identifications can be imperative ( e. Taxonomic identification of biological materials can be achieved through DNA barcoding, where an unknown “barcode” sequence is compared to a reference database. ![]()
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